Table 2. Yield of precipitate produced from reaction mixture with laccase in (+)-catechin aqueous solution on reaction condition

Enzyme reaction condition Yield (%)
Reaction temp. (°C) 30 65.3
40 70.1
50 58.7
Reaction pH 3.0 61.3
5.0 63.0
7.0 68.7
9.0 27.4
Enzyme amount (%) 0.5 33.4
1.5 67.5
3.0 70.1
5.0 83.7
Substrate amount (%) 0.2 92.5
0.5 67.5
1.0 21.3
3.0 14.9
Substrate (+)-Catechin 64.7
Epicatechin 65.2
EGCG 61.1
Removed-GA1) 58.0
Removed-GA; removing gallic acid from green tea catechin compounds by enzymatic treatment.